Antibacterial Activity and Time-kill Assay of Terminalia catappa L. and Nigella sativa L. against Selected Human Pathogenic Bacteria

The current investigation aims to test the susceptibility of human pathogenic clinical isolates and MTCC strains leaf seed extracts Terminalia catappa Nigella sativa. Disc diffusion assay, micro dilution assay minimum Bactericidal Concentration investigated bacteria extracts. active extract was subjected phytochemical screening, separation phytochemicals by Thin Layer Chromatography, bioactivity guided Time- kill assay. Acetone methanol T.catappa revealed, significant inhibition origin Staphylococcus aureus followed Proteus vulgaris aureus, Salmonella typhi, Pseudomonas aeroginosa Bacillus subtilis. sativa inhibited growth Staph.aureus strain Staph.aureus, typhi B.subtilis. Minimum inhibitory concentration for all reported in range 5000μg/ml 9 μg/ml T. extract. Most sensitive being isolate Staph. vulgaris. bactericidal found be between 625μg/ml. Phyto-chemical analysis have dominated polyphenols (Terpenoids, steroids, flavonoids, flavones, saponins tannins) N.sativa recorded presence alkaloids, proteins oils fats. TLC profiling acetone revealed many antibacterial bands. Bands having Retention factor 0.47 0.52 were against bacteria. Time carried out first time. exhibited dose dependent bacteriostatic activity isolates.